phosphorylated perk (pperk) antibody  (Wanleibio)

Journal: Ecotoxicology and environmental safety

Article Title: Biocorona formation contributes to silver nanoparticle induced endoplasmic reticulum stress

doi: 10.1016/j.ecoenv.2018.11.107

Figure Lengend Snippet: RAEC were exposed to AgNPs (20 µg/ml) with or without a BC for 3hrs and protein expression was measured by Western blot analysis. Densiometric analysis was performed using ImageJ comparing 20nm (light gray) and 100nm (dark gray) AgNPs with a BC (BSA-bovine serum albumin, FBS-fetal bovine serum, HDL-high density lipoprotein). (A) Images of pIREα, IREα, pJNK, and actin expression in RAEC exposed to AgNPs with or without a BC. (B) Images of BIP, XBP1, and actin expression. (C) Images of pPERK, PERK, ATF6, and actin expression. (D) Images of CHOP and actin expression. (E) Densiometric analysis of protein expression measured by western blot studies (N=3). *-statistically significant compared to control (p<0.05); #-one way ANOVA indicating a statistically significant compared to 20nm and 100nm with similar BCs.

Article Snippet: Western blots were probed using antibodies against phosphorylated-PERK (pPERK), PERK, CHOP and IRE (Cell Signaling Technology, Danvers, MA, USA); phosphorylated-IRE (pIRE), ATF6 (Abcam, Cambridge, MA, USA); and actin (Sigma Aldrich, St Louis, MO, USA).

Techniques: Expressing, Western Blot, Control

Journal: Ecotoxicology and environmental safety

Article Title: Biocorona formation contributes to silver nanoparticle induced endoplasmic reticulum stress

doi: 10.1016/j.ecoenv.2018.11.107

Figure Lengend Snippet: RAEC were exposed to AgNP (20 µg/ml) for 3 hrs. (A) Densiometric analysis of pIRE/IRE per ug/ml of Ag+ uptake measure by ICP-MS (N=3). (B) Densiometric analysis of pJNK per ug/ml of Ag+ uptake measure by ICP-MS (N=3). (C) Densiometric analysis of pPERK/PERK per ug/ml of Ag+ uptake measure by ICP-MS (N=3). (D) Densiometric analysis of CHOP per ug/ml of Ag+ uptake measure by ICP-MS (N=3). Reactive oxygen species levels were measured in RAEC by DCF while Western blot analysis was used to investigate the induction of ER stress. (E) Reactive oxygen species measurements in RAEC exposed to 20nm or 100nm AgNPs (20 µg/ml). *statistically significant (p<0.05). (F) Images of protein expression measured by Western blot analysis of RAEC pretreated with N-acetylcysteine (NAC) and exposed to 20 nm and 100 nm AgNPs for 3hrs. *statistically significant compared to cells without NAC pretreatment (p<0.05).

Article Snippet: Western blots were probed using antibodies against phosphorylated-PERK (pPERK), PERK, CHOP and IRE (Cell Signaling Technology, Danvers, MA, USA); phosphorylated-IRE (pIRE), ATF6 (Abcam, Cambridge, MA, USA); and actin (Sigma Aldrich, St Louis, MO, USA).

Techniques: Western Blot, Expressing

Journal: Diabetes

Article Title: Celastrol Reduces Obesity in MC4R Deficiency and Stimulates Sympathetic Nerve Activity Affecting Metabolic and Cardiovascular Functions

doi: 10.2337/db18-1167

Figure Lengend Snippet: Changes of the levels of gene expressions in the hypothalamus by celastrol. A–D: Expression levels of genes involved in ER stress. E–G: Expression levels of inflammatory cytokine genes. H–J: Expression levels of neuropeptides involved in homeostatic regulation of energy balance. K–M: Expression levels of genes involved in leptin signaling. N: pPERK and SERCA2 protein levels in the hypothalamus. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 by Student t test compared with vehicle group.

Article Snippet: The membrane was incubated with the following primary antibodies from Cell Signaling Technology diluted in blocking buffer overnight at 4°C: SERCA2 (#4388), total protein kinase RNA-like ER kinase (PERK) (#5683), and phosphorylated PERK (pPERK) (#3179).

Techniques: Expressing